Agrobio Industry
Topic 1
Food quality monitoring: selection, and traceability
Topic 2
The biology and physiology of molluscs and algae
Topic 3
Bacteria adaptation to the environment
Topic 4
Microorganism characterisation and selection for cheese-making
Topic 5
Mammal hormones and reproduction
Topic 6
Nitrogen transfer to natural and soilless crops
Topic 7
The microbiological quality of food
Topic 8
The sensory qualities and flavours of food
Topic 9
Technological innovation (products / process)
Topic 10
International documentary watch
     
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TOPIC 8

SENSORY AND AROMATIC QUALITY OF FOOD

Part 1
Key words :
flavours, sensory profiles, olfactometry, hedonic approach.
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The sensory and physical and chemical characterisation of the aromatic quality of fermented products (soft cheeses / cider-based products) are addressed.

Characterisation of key olfactory compounds using gas combined with olfactometry

Identification of different compounds (partnerships: INRA and ERPCB Caen laboratories)
Sensory profiles of the relevant products by a panel of experts
Statistical correlation (ANOVA, ACP) between the physical and chemical composition and the sensory characteristics
   
The Teams
15 people.
R&D 'Flavours' Team
Sensory Evaluation Team
   
Equipments
Varian CP3800 chromatograph coupled with olfactometry (SGE System)
Varian CP3800 chromatograph for routine assays (beverages, cheeses, and so on)
Delsi DI 700 chromatograph for routine assays (fatty acids)
Tekmar 2000 headspace machine
Laboratory of standardised sensory evaluation (2 x 20 booths)
Statistical software: SPAD, Statgraphics, Statitcf, and Fizz
   
Methods
Extraction: liquid-liquid, resin, vacuum distillation, and Headspace. The methods are selected according to the different food matrixes
Chromatography coupling in gas/olfactometry phase. This method can pinpoint the molecules with a sensory impact, in a complex mixture. It highlights the compounds related to product quality or those directly related to one or several sensory defects
Descriptive (qualitative and quantitative) sensory analysis: detailed sensory profiles (flavour, odour, aroma) are drawn up. Panels of experts: Ciders and must (wine) or wort (beer) Calvados (cider spirits) Dairy products (butter, Camembert)
Hedonic approaches: acceptability of the products by a consumer panel (base-panel of 3,500 individuals)
   
Application examples
Identification of the molecules creating the flavours in spirits
Establishment of a sensory profile of the main varieties of the apples sold in France
Set-up of a panel in an agrifood company to control the sensory quality of the products made by the company
   
Contacts
For general technological or scientific information

M. Bernard PICOCHE bpicoche@adrianie.org
Melle Sylvie SIBIRIL
ssibiril@adrianie.org
M. Hugues GUICHARD
hguichard@adrianie.org
ADRIA NORMANDIE
- Centre de Ressources Technologiques
Bd du 13 Juin1944
14310 VillersBocage
Tél : 33 (0)2 31 25 43 00 - Fax : 33 (0)2 31 77 49 43

   
For economic or industrial information
Normandie Développement
57 avenue de Bretagne - BP 1083
76173 Rouen Cedex 1 - France
Tel : 33 (0)2 35 03 06 04 - Fax : 33 (0)2 35 03 07 86
ndrouen@normandydev.com

Part 2
Key words :
yeast, lactic acid bacteria, ripening bacteria, spoilage bacteria, detection, identification, characterisation, selection.
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Characterisation and selection of microorganisms for the improvement of the sensory qualities of fermented products (soft cheeses and cider-based products)

Identifying, characterising and selecting the microbial strains promoting the control of dairy product fermentation (cheeses) and plant fermentation (ciders and olives)

Highlighting the flavour enhancing qualities of yeast and bacteria and the pigmentation properties of surface bacteria on cheeses
Studying and detecting the strains causing product spoilage (acrolein production or cider sickness, production of biogenetic amines in fermented products)
   
The Teams
The Microbiology Unit at the Research and Development Department has two project leaders (1 microbiology engineer and 1 Doctor in Molecular Biology) and 3 research assistants.
   
Equipments
Various equipment for yeast and bacteria cultures, freeze-dryer, microscopy, centrifuges, DNA electrophoresis, spectrophotometer, hybridisation oven; DNA amplifiers; computer systems for analysis and image processing, colorimeter, gaseous phase chromatography, deep-freezer, and cryopreservation machine
   
Methods
Maintenance, enrichment and enhancement of strain collections (biodiversity)
Specific counting, isolation, identification and characterisation of microorganisms with technological properties, and spoilage microorganisms
PCR, PCR-RFLP, DNA/DNA hybridisation, DGGE/TGGE, molecular probes, FTIR
Culture in model environment and on food matrix
Colour measurement, assays of the flavour compounds, study of the technological qualities (acidification kinetics, fermentation speed, bacteriophage resistance, proteolysis, viscosity, and so on)
   
Application examples
Bacteria selection for the development of flavours in cheeses
Identification of the microorganisms to blame for a taste accident in cider
Manufacture of ferments for industrial tests at cheese dairies
FTIR identification of lactic acid bacteria and ripening bacteria, and set-up of a strain bank
   
Contacts
For general technological or scientific information

Mr. Bernard PICOCHE
ADRIA NORMANDIE - Centre de Ressources Technologiques
Bd du 13 Juin1944
14310 VillersBocage
Tél : 33 (0)2 31 25 43 00 - Fax : 33 (0)2 31 77 49 43
bpicoche@adrianie.org

   
For economic or industrial information
Normandie Développement
57 avenue de Bretagne - BP 1083
76173 Rouen Cedex 1 - France
Tel : 33 (0)2 35 03 06 04 - Fax : 33 (0)2 35 03 07 86
ndrouen@normandydev.com