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Not only does the research address the acquisition of new fundamental
knowledge about the endocrinology of animal and human reproduction
but also the enhancement of said knowledge through the search
for efficient zootechnical strategies. Research focuses on two
fields:
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The study of the molecular and cellular mechanisms
regulating the expression of the aromatase gene (an enzyme that
converts male or androgynous hormones into female hormones or
estrogens) in gonad cells |
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The role of estrogens in the gonads:
- The study of any relations between estrogens and programmed
cell death
- The influence of endocrine disrupters (xenobiotics) on reproductive
functions |
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| The
Teams |
"Aromatase and estrogens in
mammalian gonads"
EA 2608 - Unit under contract with INRA (the national institute
for agricultural research). The team has a full-time staff of
13 (3 Professors, 8 Senior Lecturers, 2 CNRS researchers), 8 doctoral
candidates, and 6 administrative and technical personnel (IATOS). |
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| Equipments |
| The special equipment can be found
at the laboratories of protein and steroid biochemistry (chromatographs,
electrophoresis machines, centrifuges, scintillation counters,
and so on) partnered with the equipment at the laboratories of
Molecular Biology (DNA/RNA amplifier, DNA sequencer, image analyser,
and so on) and with the equipment belonging to the laboratories
of Cellular Biology (fluorescent microscopy, CO2 incubators, hoods,
and so on). Equipment for cell and tissue cryopreservation is
also available. |
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| Methods |
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Bioassays on cell cultures for gonadotropins |
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Radioimmunoassay (RIA) of steroids |
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Gene cloning and study of their expression and transfection |
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Detection of messenger ribonucleic acids (RNA) and
related proteins |
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RT-PCR (Reverse Transcription Polymerase Chain Reaction)
technique used for special RNA assays |
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Quantification and analysis of proteoglycans |
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Lipoprotein assay and analysis |
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Gonad cell purification |
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Detection and identification of pathogen mutations
in humans |
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Impact studies of endocrine disrupters on reproductive
functions (applications in toxicology and pharmacology) |
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Cryopreservation and quality of reproductive cells |
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| Contacts |
| For general technological
or scientific information |
|
M. Carreau (Professeur)
Institut de Biologie
Fondamentale et Appliquée (IBFA)
Université de Caen
Tél : 33 (0)2 31 56 53 20 - Fax : 33 (0)2 31 56 53 20
carreau@ibba.unicaen.fr
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| For economic or industrial
information |
|
Normandie Développement
57 avenue de Bretagne - BP 1083
76173 Rouen Cedex 1 - France
Tel : 33 (0)2 35 03 06 04 - Fax : 33 (0)2 35 03 07 86
ndrouen@normandydev.com |
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