Agrobio Industry
Topic 1
Food quality monitoring: selection, and traceability
Topic 2
The biology and physiology of molluscs and algae
Topic 3
Bacteria adaptation to the environment
Topic 4
Microorganism characterisation and selection for cheese-making
Topic 5
Mammal hormones and reproduction
Topic 6
Nitrogen transfer to natural and soilless crops
Topic 7
The microbiological quality of food
Topic 8
The sensory qualities and flavours of food
Topic 9
Technological innovation (products / process)
Topic 10
International documentary watch
     
Click on to download the topic PDF file.
     
     
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General funding
Project follow-up
Training programs
Corporate user services at operations site
Technology transfer to companies: www.gravir.org and www.iaa.cra-normandie.fr
     
     
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or send an email to: ndrouen@normandydev.com or ndcaen@normandydev.com

TOPIC 4

THE CHARACTERISATION AND SELECTION OF MICRO-ORGANISMS FOR CHEESE-MAKING

Part 1
Key words :
Geotrichum candidum, Lactobacillus sp., Lactococcus sp., biodiversity, cryopreservation, raw milk, soft cheeses, typical flavour and taste, terroir, ecosystems, conservation, probiotics.

The Laboratoire de Microbiologie Alimentaire (LMA, Laboratory of Food Microbiology, UCBN), a unit under contract with INRA, works mainly on the microbial ecosystems of raw milk and soft cheeses from Normandy. The Laboratory focuses mainly on the two following fields:

The study - essentially from an ecological outlook - of the technologically useful microflora, with a focus on their biodiversity and the contribution of this biodiversity to the typical flavour and taste of cheeses
The improvement of the ex situ (deep-freezing) and in situ preservation of microbial strains, with a view to initiating the preservation of the microbial consortia from the environment of dairy herds
   
The Teams
Laboratory of Food Microbiology E.A 3213 U.S.C INRA
Twenty to twenty-five people (5 research teachers, 3 technical and service personnel, 3 seconded researchers from companies, 8 research students (postgraduates, doctoral candidates, European contract, and students with advanced masters), 6 other interns
   
Equipments
Polymerase Chain Reaction (PCR) amplifier:
Pulsed Field Gel Electrophoresis (PFGE) equipment and Temperature Gradient Gel Electrophoresis (TGGE) equipment
High Performance Liquid Chromatography (HPLC) equipment, epifluorescent microscope
   
Methods
LMA expertise relies on the use of methods and technologies for the following applications:
The isolation, identification, and characterisation of bacteria, yeast, and mould for dairy purposes
The adaptation of microorganisms for deep freezing
The analysis of microbial genome diversity
The study of the probiotic potential of microorganisms for dairy purposes
The preparation of the doses for cheese dairy leaven
The establishment and management of microorganism collections
The cell cultures of microorganisms and enterocytes
Electrophoretic technologies (PFGE, TGGE, proteins, and so on)
HPLC assays
Epifluorescent microscopy
   
Contacts
For general technological or scientific information
Mme Micheline GUEGUEN
Laboratoire de Microbiologie Alimentaire - Université de Caen

Tél : 33 (0)2 31 56 56 98
gueguen@ibba.unicaen.fr
   
For economic or industrial information
Normandie Développement
57 avenue de Bretagne - BP 1083
76173 Rouen Cedex 1 - France
Tel : 33 (0)2 35 03 06 04 - Fax : 33 (0)2 35 03 07 86
ndrouen@normandydev.com

Part 2
Key words :
FTIR spectroscopy, bacterial classification and identification, lactic acid bacteria, corynebacteria, micrococcaceae.

The identification and characterisation of bacterial strains using Fourier Transform Infrared (FTIR) spectroscopy

   
The Teams
The Physical Chemistry and Biotechnology Research Team has 11 research teachers (2 Professors and 9 Senior Lecturers), 2 technical and administrative personnel, 3 doctoral candidates
   
Equipments
NICOLET 250 and THERMO OPTEK Avatar 360 FTIR spectrophotometers
Special spectral libraries of lactic acid bacteria and ripening bacterial flora for a strain identification database
   
Methods
The FTIR spectrum that is directly recorded onto a dried sample in compliance with a strict protocol is special to a bacterial individual and matches the vibrational characteristics of all its cell components. The computer processing and discriminatory analysis of the spectrum data makes it possible to classify the bacteria according to genus, species and strain. The technology has been applied to the identification, differentiation and classification of bacteria for cheese making purposes: ripening flora (corynebacteria and micrococcaceae) and lactic acid flora (Lactobacillus, Leuconostoc, Lactococcus and Streptococcus).
   
Contacts
For general technological or scientific information
Caroline AMIEL
Tél : 33 (0)2 31 56 71 19
C.Amiel@iutcaen.unicaen.fr
Laurence MARIEY
L.Mariey@iutcaen.unicaen.fr
   
For economic or industrial information
Normandie Développement
57 avenue de Bretagne - BP 1083
76173 Rouen Cedex 1 - France
Tel : 33 (0)2 35 03 06 04 - Fax : 33 (0)2 35 03 07 86
ndrouen@normandydev.com